megatomi.com for Dummies
megatomi.com for Dummies
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Our new Apparent+ tissue clearing procedure is the sole technique that delipidates samples without having change in morphology and with negligible effect on structural integrity.
Our flip-key SmartBatch+ method combines electrophoretic tissue clearing and immunolabeling into one substantial-throughput machine.
Antibodies may well consider weeks to diffuse by way of only a few millimeters of tissue, by using a steep labeling gradient from surface to Main.
Working the CLI directly from a Gradle process is not at present supported. A distribution needs to be produced by means of gradlew :j2d-cli:distZip to produce a zip file containing all the things needed to run.
SE takes advantage of a rotational electric discipline to disperse highly electromobile molecules (for example antibodies or surfactant micelles) in the course of a porous sample without harmful electrically billed buildings throughout the tissue. This enables 2-4 working day clearing of intact organs,
Our preformulated EasyIndex Alternative raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables mild penetration to the sample and makes certain the acquisition of significant-resolution, in-aim graphic data.
Megatome is usually a novel microtome that permits for high-precision sectioning of a wide range of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with minimum tissue destruction and knowledge reduction.
It’s time to enhance your microtome to megatomi.com Megatome. With precise high-frequency slicing for an unmatched array of sample sizes and kinds – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for various programs.
The SmartSPIM light-weight sheet microscope supplies unparalleled resolution and acquisition speed all over intact tissue volumes, even though its modular design and style allows you to up grade and personalize individual parts.
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Defend avoids the variability of hydrogel embedding and the data loss from PFA preservation, safeguarding specimens for many rounds of processing.
Leverage the Obvious+ tissue clearing strategy, in conjunction with eFLASH and patented stochastic electrotransport systems, to promptly distinct and label complete organs. Critical highlights and characteristics include things like:
Our novel Protect tissue preservation procedure forms intramolecular bonds utilizing polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.